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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      ) d 07<d 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 R1fPPJ         4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0dSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ02<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f*J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@337    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell) x^fff               d 0.3d 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell <6ffl          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 R1fPPJ         4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0dSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ02<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f*J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@337    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell) x^fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell <6ffl          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 R1fPPJ         4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0dSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ02<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f*J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@337    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell) x^fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell <6ffl          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 R1fPPJ         4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0dSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ02<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f*J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@337    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell) x^fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell <6ffl          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 R1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R D8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0dSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ02<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f*J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@337    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell) x^fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell <6ffl          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 R1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R D8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0dSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ02<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f*J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@337    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell) x^fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell <6ffl          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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 t40ڀ΀KQembryo cloning  (WSOY(ue'`A~kve_NuN|RW Oir([hQv Tvڀ΀vǏ z0$;#F    R60ڀ΀~ހ(embryonic cell) ڀ΀uSˆt~ހbQ~ހV~ހ0,*%!L       70ڀ΀r^~ހ(embryonic stem cell) Nڀ΀Q~ހVbSYukzRyQegv(WSOYePXkNwQ ghQ'`v~ހ0 6F)2   ,   80~ހhQ'`(cell totipotency) ~ހ@bwQ gv1uNy~ހRSb_buir@b gv~~~ހSbukz(WQ vR0&FZ*J     ,  t90~ހY'`(cell multipotency)~ހ@bwQ gv1uNy~ހRSb_b$Nyb$NyN N~ހb~~vyr'`0,;>    !  N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0@<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *         !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{}~% "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell) x^fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. [{ uSNuSk~ހ YTSO \hQuSNuSk~ހ YTSOR~0 A~|~ހB\[teN'}[,N,:N4^6B\,ހ(GWS B~|~ހB\\,N,:N2^4B\,ހ(GWS C~|~ހB\ NhQb:NuS0S bA B~uSNuSk~ހ YTSO(uNbqW{Q0z0i2ffff#n                                 D.uSk~ހbqt[ \W{Q19^22hv[ruSk~ހR+R(uD-hankmnm3M,>eeQ+T g0.3%f(xvvD-hankmQ,W{Q{-Nu[10min (u150mvk~st{{{9TSbNdS|~ހ,\huSk~ހ0YgQs,{NgSOfuSk~ހ]W{Qbq SNۏL>f_d\O0<Z f:                           &  ba$OSO~ހQY Ǒ(u>f_RrRbvmSvel \VڀgMRvڀ΀RbQNbbUS*Nڀ΀~ހ0(2 )&  #  |b$uSk~ހS8h >f_d\O =\S\uSk~ހ8hSc0S8hd\OT W{Q{Qu[30min b_`~g[te(uNg^KQڀ΀067 3             0 0 xd$͑gڀSOYW{Q \;mSv͑gڀ>eeQW{Qm-N (W38.5! 5CO2 qTn^vW{Q{-NW{Q048hT‰[uSˆ`Q06 34               e$͑gڀy i Ǐ^Kb/gvel \S0RQh9iڀbVڀv͑gڀQhZڀbVڀ yeQ0RSSOk[rP[[ OY Z Nr0SSOk[rYNcuST,{7 8)Y 0SOY͑gڀS`Q(VP KV  <       10.SO~ހ8hy i 1 i_ Ry~~bUS*N~ހ \US*NSO~ހl\eQS8huSk~ހ-N v^Ǐo;m0TOvQRˆ0Sb_begڀ΀Nvb/g y:NSO~ހ8hy i0<SZ 33CJ     =  2 {kNsTuusؚ 1uN/fe'`A~k KQTNNN'` g[WVff>f'` [}T0uuf%N͑Su0Y  Dolly N;m5\ N`%N͑sQp0(Wd\OǏ z-N S[~ހ b\o(WvqS[0 3 NvKQ g̀N>yOf[T&Otf[0FzZ f\f       &         N r^~ހ 1.i_ r^~ހstem cell,SC)/fN{|wQ gePbfeRv~ހ YNu\Ny{|Wv0ؚ^RSvP[N~ހ (WSOY'YϑibX0QX[ N1YvQS gyr'`0f M33ff<V       <  2.R{| 1 9hncSuf[egnR:NN N$N'Y{| `$ ڀ΀r^~ހ /fNegڀ΀Q~ހVbSYuk~ހ~SOYRSb6RW{Q[{ RyQegvwQ gShQ'`v~ހ0[eSNavQ[~ހN7h(WSOYW{Q0X     '   Yr^~ހ wQ gwQ gbfeTNuYy{|W~ހvR FOtS'N1YNSb[te*NSOvR S\oS0RN[vP6R0YX[(WNӚv @r^~ހSRSQ\12y@~ހ0LOO>   A   C' ^ USr^~ހwQ gbfeR STUSNeTRS NuNy{|W~ހ0Y!-Nvb~~~ހI{040) 0 DE( 3. r^~ހ] z 1 [IN SOYRyW{Qr^~ހ v^[vQۏLTyd\Ov]zSO| vQvv/fxvzvQuirf[yr'` ^(uNW@xxvzT4N^lu0:G  333J     + F) 2 xvzV 1 Ry0~S0KQ0OX[r^~ހ ^zr^~ހ| 2 xvzr^~ހvuirf[yr'` Yb_`0u0h0RSI{yr'`Nĉ_ 3 PRr^~ހ xvz~ހRSb_b~~SSuirf[ 4 r^~ހNlWV 5 4N^^(u luuu ZnZ 33ff ff f       !          2 ڀ΀r^~ހvyr'` ~ހhQ'`(cell totipotency) ~ހ@bwQ gv1uNy~ހRSb_buir@b gv~~~ހSbukz(WQ vR0 a$ wQ gePXkvR0~ " -"  f-B$ '   R3 )R(u~ހ8hy iڀ΀Ryڀ΀r^~ހ \bSO~~~ހbڀ΀~ހ0ڀ΀r^~ހl\eQS8huSk~ހ-----o;m T---SOYW{Qb_begڀ΀----SOYW{QbVڀ---- mSQ~ހV ----RyQQ~ހV~ހ ----cyNb~~~ހr{QB\W{Q---ڀ΀r^~ހƖ=----cQ*gRSUSKQ~ހV---- yce -----QcyibX0zPP3f    ,                              0 0OQ  `$\ ΀?QvSNYt eY{kY Zk (u75%R|mky (uYyKb/ghVhẽSQ΀?Q nNeAPBS-N Sd4Y0VSQ (ueAPBSmm3^5!k Sd@m Q(u     [S 3 uNlWVRir W{Qڀ΀r^~ހ v^(uYnWVlgڀ΀r^~ހ ~Ǐ[{ SSlWV~ހ Ndk:NPge KQRir SSlWVRir09eoRir 6RYuirS^hV00V 33F2   ? \1 ~4 ^zoirċN!jW~ހ 5 xvz~ހRS0~~b_bvW,gĉ_ 6 xvzWVvR :1Y zS 7 6RYRir!jW0n@ 3333 33 3333l          ]^_T 44 @{TQv~ހ 5 \~ހ0 6 ~ހ\fffT        `aU 2 [T[ڀ΀r^~ހRS:NR'`hV[vMRagN0:g6R Nn gяvxvzhf S\r^~ހl\0R`uMO r^~ހbRS S%cO Y\O(u0FOhV[vb_b YBg )R(ur^~ހN hV[N͑ S܏ <^?$  ] bV B3 ڀ΀r^~ހv[hQ'` 4 mS&Ot0>yO0S_vN0""!<     cdڀ΀r^~ހSvQW{Q Ɖ*  efW N20SSO~ހreceptor cell  cSvvWVv~ހ0$(L       g2 3.vvWVtargetic gene  vvWV_Ny:NvhWV /fcwQ gyr[R [eQ0RRirWV~bۏLW Od\OvWV0&GZ-0    3 hX \40WV~gene group  /fcRir*NSObSO@bS+Tv@b g{|W WVv;`T0./ZEAL       ijY X6.lStransformation :vvWVޏcNgrSOWV~ v^ۏLhvsa04-H      kZ 67.}SOvector ::d&^vvWVvDNAGrk0 , `        l3 8.lWVRirtransgenic animal  /fc[RirWV~ۏLW Od\O[eQvvWVb9eSS gWV~g v^Oُy9eSvW OOo`Y(WTN3z[W OvRir y:NlWVRir0 <`A>    4 m[ 9.RiruirS^hVbiology reactor by animal production  YN@m0sNAlbvQ[NT-NcSTuNwQ g_ؚN t2 bS bSOl 6RYY|P[bSO |e(W|m-NR gY|P[bSO Syr_'`@gmpY|P[0 3 ߘirl2;f" f                p2|P[[{ l 1 [^h^y_l 9hncx|P[TY|P[[^v]_ Ǒ(uh^y_l\x|P[TY|P[Ry029f"             ? b2 5ull 9hncx|P[TY|P[hb:d&^5uwN(ϑv]_ Ǒ(u5ull\x|P[TY|P[Ry0$2 f'             @ F3 Am_~ހhVRyl 9hncx|P[TY|P[DNA+Tϑv]_ (uDNAyr_'`ge[|P[ۏL;mSOgr (uoIQ_ggq\grTv|P[ SIQ:_v:Nx|P[ SIQ1_v:NY|P[ aIQ|~\ُyIQOS O~{:g {:gc6REQ5u hV ~SIQ:_vx|P[&^ck5uw SIQ1_vY|P[&^5uw S_&^5uwv|P[Ǐ5u:We 1\S\x|P[TY|P[Ry02 f.             !    #          A (2 SRYWVchKml )R(uSRY -PCRZTv_S^ SOYibXSRY WVc0cSڀ΀~ހDNA NSRYWVvNkxW:N_ir Nڀ΀~ހDNA:N!jg ibX+TSRYWV gڀ΀~ހDNA [ibXNirۏLSouthernBgN Qs3'` :NĖ'`ڀ΀ Qs4'` :N̖'`ڀ0< f                       B B4 gIQMQul A:NH-YbSUSKQbSO B:N(ugIQhvq\b\ U Blˆ}v C :NĖ'`ڀ΀~ހH-YbS0 \AReQW{Qm-NW{Qڀ΀ b_bA-C YTSO Q\BReQڀ΀~ހhb Ė'`ڀ΀Sb_bB- A-C YTSO ̖'`ڀ΀ NSb_bB-A-C YTSO0 (WgIQ>f_\ N B- A-C YTSO>fgIQ >fgIQvڀ΀:NĖ'`ڀ΀0Z GfC6C"G33$CCV                              ` 62.R{| 1) yQL]TSO 2) yL]TSO\f fH      a 3 ڀ΀r^~ހembryonic stem cell ESC (4) xu΀$v~ހembryonic carcinoma cell,ECC). xNZZff           dC V2. \Y*NOSO~ހl\eQSSOڀ΀-Nb\$N*Nڀ΀Q~ހVZƖ(WNw ňNzzf&^-N0$, ( D H3.W{Qڀ΀ 37!,5%CO2,qTn^ ~ހW{QmDMEM). 6$H         44.ۏLL]TSOt[ 1 krI{YWt[ L $f f0      b 2 uSchY TRvt[((ubJTWVh ) a:~rgIQˆ}vWVGFP ; b:-JSsN|vWV(Lac Z ; c:/l }YNplyvWV(CAT); d:Nuo }WV(hGH)f                           E Z3 RP[BgN  4 KmNՋ 40~100%krL]TB& NOڀegnT|@& 0P. f f$            c 5. ۏLڀ΀y i0$  $    N aINThe function of production of Mammalian chimeras 1. uNlWVRir 2. bQeoqSz gRir 3. WeTy 4. xvzWV~gNRvsQ| 5. uNN ~~hV[ z334    1               *wƋbU\Procedure of IVF($  FHyper ovulation Egg Retrieval Artificial Insemination Embryo TransferG" ZG G F N aIN 1. 'YE^^cؚk\uA~kR EQRS%cOok\uvA~k\oR 2. /fڀ΀] z uirb/gSU\vW@x 3. :NN{|;Sf[vSU\cOb/g/ec0jN33T        .2.[k\ud\O 1 ;mSOǑuS pecuS-----yT\b/gSuS----V6euSk~ހ-------W{QuSk~ހ-------OvQbq0 2 uS]ǑuS SuS]-----mmuS]-------NuSlb8TuSk~ހ-----SOƉ>f_\yS0mmuSk~ހ------bqW{QuSk~ހʑ>e,{NgSO 0ff6fI                          G T3. |P[NuSP[qQW{Q |P[TuSP[(WSOY[bS|Ǐ z 4. egڀ΀W{Q02+  0    H  2.[{ uSNuSk~ހ YTSO \hQuSNuSk~ހ YTSOR~0 A~|~ހB\[teN'}[,N,:N4^6B\,ހ(GWS B~|~ހB\\,N,:N2^4B\,ހ(GWS C~|~ހB\ NhQb:NuS0S bA B~uSNuSk~ހ YTSO(uNbqW{Q0{Z fff$\                               I (4. uSk~ހbqt[ \W{Q19^22hv[ruSk~ހR+R(uD-hankmnm3M,>eeQ+T g0.3%f(xvvD-hankmQ,W{Q{-Nu[10min (u150mvk~st{{{9TSbNdS|~ހ,\huSk~ހ0 YgQs,{NgSOfuSk~ހ]W{Qbq SNN|P[qQ TW{Q00 .                          , J f6.S| \|P[TuSP[(Wm-NqQW{Q |P[TuSP[[bS|\O(u b_bTP[ uSˆT b_begڀ΀0.4ZE.A&   K p7.egڀ΀W{Q\S|uSnNTCM199+@n+mRirvW{Qm-N SOYW{Q S_ڀ΀SVڀge eSNO(u0Z9Z EA EfAAV         wƋbU\Ջ{tZ?Qb/g, D& $  :,{NNՋ{tZ?Qin vitro fertilization ,IVF SOYS| ,{NNՋ{tZ?Q(intracytoplasmic sperm injection, ICSI US|P[uS~ހFmQl\) ,{ NNՋ{tZ?Qpre-implantation genetic diagnosis, PGD ڀ΀ ieQMRʋe).          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This information is helping parents choose which embryos they want--and which to reject as unhealthy, or merely undesirable. $! 2  Undesirable Embryos(  iDisease Free Embryos Frozen in storage Donated to infertile couples Donated to stem cell research/usage ,Tji   7Disease Carrying Embryos Donated to research Discarded  87  l,{ NR \ONN` 33  N0(WQ Nd"} [~bRirڀ΀r^~ހxvzgebgvVGr0 N0N\~:NUSMO gDe g'`A~k/f6qۏSv~g KQ/f&T,g1\/fP $FE F ,{VR T 33  @8 __WPP6pOp   0  ((  r  S 1 Tt  Z  s 2A 0VGr5s '}  s fA< 001000000000000119081078348414g ~  H jJ?"` -  N  6 ޽h? ff3333f^  0  (    s *HJf <Tt7  *  s LA" 03AKD6P7MMTYIINDF:<@# 0 0N  6 ޽h? ff3333f  0   (  r  S A e  n  s FA 0~ހ8hy i:yaVKNOSO~ހ2W^N  6 ޽h? ff3333f  0 @8 (  r  S I ,    s dA: 0female_reproductive_system_3  N@K ?"  S  RuS{f  jB  B !Ԕ? ~   NL ?" l RP[[҉f  jB @ B !Ԕ?)   BPR ?" j $ T SSOW{Ql33  N  6 ޽h? ff3333f  0 tl (  r  S T`    j  s BA  0chap5_fig1b    Ha ?"   5 Ty iڀ΀f  N  6 ޽h? ff3333f  0 | (  r  S  j _  \  s 4A!  0A~kR2 N  6 ޽h? ff3333f 0  (     6 2  f3.ڀ΀RrRlKQRirxvzۏU\progress on animal cloning by splitting embryo 1 1904t^ Spemann\نvڀ΀ۏLRrR _N TڀSuTN0 2 1930t^ Pinus\TQP[vUS*N2-~ހuSˆtW{Qb:NSOy\vNVڀ 3 1970t^ MullenRy\ 2-~ހڀ΀uSˆt _ TuSSuTN0@t '330''f#  'f#'f#8  .                        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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0@<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell) x^fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -  §çħŧƧǧȧɧʧ˧̧ͧΧϧЧѧҧӧԧէ֧קا٧ڧۧܧݧާߧK  5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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%e2[\uA~kf[ Animal Reproduction*<0  ;NYe^[z Yec0ZSX,  f,{ASz RirA~kuirb/g'0  ,{NR f[`NcWS N0f[`Nvh Ǐ,gzYef[ Of[u 10cc 1 SOYS|vi_SaIN uSk~ހvǑƖTbqW{Q |P[v_N SOYS|vW,g z^ egڀ΀vW{QNOX[b ZTZ G(33G$$:$        7 2 RirKQvi_ RirKQvy{| RirKQb/g0elN z^ 3 lWVRirvi_, Rir'`+Rc6Rvi_Nel, RirL]TSOvi_ RirL]TSOuNel r^~ހ ڀ΀r^~ހ ~~r^~ހ0``l      <k $20t 1 ڀ΀y ivaIN RS|b/gaIN lWVRiruNvaIN 2 lWVRirb/gv;Ns,'`+Rc6Rb/gNaIN,RirKQb/gvaIN 3 RirL]TSOvaIN,ڀ΀r^~ހvRy0KQNW{Q ڀ΀r^~ހ[RS ~~r^~ހvRyNW{Q 30N ڀ΀r^~ހN~~r^~ހv^(uVPGuC GC        !   .   g pN0f[`N͑pNp N ͑p SOYS|0RirKQ0ڀ΀r^~ހ 0 N p Rir'`+Rc6R RirL]TSOuN 0~) G,E33ACE33A&  4 h  N0f[`Nel 10Xw,T yg` ÍS 20TgDe w[b\ON 30R\~ N\~:NUSMOSRvsQx]\O dQbJT SR0 @LG,EAT         i pV0Yef[W,gs 10Xc f[`N 20T Y`N [b\ONS` 30Tx wdQbJT 40X B8 G$/Al             j,{NR XYef[ 633     tN0RirKQb/gThe technology of animal cloning N NRirKQb/gvsQvW@xtThe Basic theory on technology of animal cloning 10KQclone cloning : KQsS:N Y6Rva`0uirf[ N@bvKQ/fc Y6RNuW Oir(T'`r[hQv TvRP[0~ހTuir*NSOvǏ z0b$#f333f4     0        4  2.RP[KQmolecule cloning  )R(uRP[uirf[b/gTel(WSOYibXT Y6R'YϑvwQ g[hQv T~gTRvWVGrk0,F(>    '  v3.~ހKQcell cloning  (WSOY(ue'`A~kve_ibXNwQ gv T~gTRvN~ހvǏ z0$<">     !  t40ڀ΀KQembryo cloning  (WSOY(ue'`A~kve_NuN|RW Oir([hQv Tvڀ΀vǏ z0$;#F    R60ڀ΀~ހ(embryonic cell) ڀ΀uSˆt~ހbQ~ހV~ހ0,*%!L       70ڀ΀r^~ހ(embryonic stem cell) Nڀ΀Q~ހVbSYukzRyQegv(WSOYePXkNwQ ghQ'`v~ހ0 6F)2   ,   80~ހhQ'`(cell totipotency) ~ހ@bwQ gv1uNy~ހRSb_buir@b gv~~~ހSbukz(WQ vR0&FZ*J     ,  t90~ހY'`(cell multipotency)~ހ@bwQ gv1uNy~ހRSb_b$Nyb$NyN N~ހb~~vyr'`0,;>    !  N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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'YE^^cؚk\uA~kR EQRS%cOok\uvA~k\oR 2. /fڀ΀] z uirb/gSU\vW@x 3. :NN{|;Sf[vSU\cOb/g/ec0jN33T        .2.[k\ud\O 1 ;mSOǑuS pecuS-----yT\b/gSuS----V6euSk~ހ-------W{QuSk~ހ-------OvQbq0 2 uS]ǑuS SuS]-----mmuS]-------NuSlb8TuSk~ހ-----SOƉ>f_\yS0mmuSk~ހ------bqW{QuSk~ހʑ>e,{NgSO 0ff6fI                          G T3. |P[NuSP[qQW{Q |P[TuSP[(WSOY[bS|Ǐ z 4. egڀ΀W{Q02+  0    H  2.[{ uSNuSk~ހ YTSO \hQuSNuSk~ހ YTSOR~0 A~|~ހB\[teN'}[,N,:N4^6B\,ހ(GWS B~|~ހB\\,N,:N2^4B\,ހ(GWS C~|~ހB\ NhQb:NuS0S bA B~uSNuSk~ހ YTSO(uNbqW{Q0{Z fff$\                               I (4. uSk~ހbqt[ \W{Q19^22hv[ruSk~ހR+R(uD-hankmnm3M,>eeQ+T g0.3%f(xvvD-hankmQ,W{Q{-Nu[10min (u150mvk~st{{{9TSbNdS|~ހ,\huSk~ހ0 YgQs,{NgSOfuSk~ހ]W{Qbq SNN|P[qQ TW{Q00 .                          , J 6.S|(fertilization) \|P[TuSP[(Wm-NqQW{Q |P[TuSP[[bS|\O(u b_bTP[ uSˆT b_begڀ΀0NCZEE.A@     K p7.egڀ΀W{Q\S|uSnNTCM199+@n+mRirvW{Qm-N SOYW{Q S_ڀ΀SVڀge eSNO(u0Z9Z EA EfAAV         wƋbU\Ջ{tZ?Qb/g, D& $  :,{NNՋ{tZ?Qin vitro fertilization ,IVF SOYS| ,{NNՋ{tZ?Q(intracytoplasmic sperm injection, ICSI US|P[uS~ހFmQl\) ,{ NNՋ{tZ?Qpre-implantation genetic diagnosis, PGD ڀ΀ ieQMRʋe).          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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{}~   % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. [{ uSNuSk~ހ YTSO \hQuSNuSk~ހ YTSOR~0 A~|~ހB\[teN'}[,N,:N4^6B\,ހ(GWS B~|~ހB\\,N,:N2^4B\,ހ(GWS C~|~ހB\ NhQb:NuS0S bA B~uSNuSk~ހ YTSO(uNbqW{Q0z0i2ffff#n                                 D.uSk~ހbqt[ \W{Q19^22hv[ruSk~ހR+R(uD-hankmnm3M,>eeQ+T g0.3%f(xvvD-hankmQ,W{Q{-Nu[10min (u150mvk~st{{{9TSbNdS|~ހ,\huSk~ހ0YgQs,{NgSOfuSk~ހ]W{Qbq SNۏL>f_d\O0<Z f:                           &  ba$OSO~ހQY Ǒ(u>f_RrRbvmSvel \VڀgMRvڀ΀RbQNbbUS*Nڀ΀~ހ0(2 )&  #  |b$uSk~ހS8h >f_d\O =\S\uSk~ހ8hSc0S8hd\OT W{Q{Qu[30min b_`~g[te(uNg^KQڀ΀067 3             0 0 xd$͑gڀSOYW{Q \;mSv͑gڀ>eeQW{Qm-N (W38.5! 5CO2 qTn^vW{Q{-NW{Q048hT‰[uSˆ`Q06 34               e$͑gڀy i Ǐ^Kb/gvel \S0RQh9iڀbVڀv͑gڀQhZڀbVڀ yeQ0RSSOk[rP[[ OY Z Nr0SSOk[rYNcuST,{7 8)Y 0‰[SOY͑gڀS`Q0(YP N  <          10.SO~ހ8hy i 1 i_ Ry~~bUS*N~ހ \US*NSO~ހl\eQS8huSk~ހ-N v^Ǐo;m0TOvQRˆ0Sb_begڀ΀Nvb/g y:NSO~ހ8hy i0<SZ 33CJ     =  2 {kNsTuusؚ 1uN/fe'`A~k KQTNNN'` g[WVff>f'` [}T0uuf%N͑Su0Y  Dolly N;m5\ N`%N͑sQp0(Wd\OǏ z-N S[~ހ b\o(WvqS[0 3 NvKQ g̀N>yOf[T&Otf[0FzZ f\f       &         N r^~ހ 1.i_ r^~ހstem cell,SC)/fN{|wQ gePbfeRv~ހ YNu\Ny{|Wv0ؚ^RSvP[N~ހ (WSOY'YϑibX0QX[ N1YvQS gyr'`0f M33ff<V       <  2.R{| 1 9hncSuf[egnR:NN N$N'Y{| `$ ڀ΀r^~ހ /fNegڀ΀Q~ހVbSYuk~ހ~SOYRSb6RW{Q[{ RyQegvwQ gShQ'`v~ހ0[eSNavQ[~ހN7h(WSOYW{Q0X     '   Yr^~ހ wQ gwQ gbfeTNuYy{|W~ހvR FOtS'N1YNSb[te*NSOvR S\oS0RN[vP6R0YX[(WNӚv @r^~ހSRSQ\12y@~ހ0LOO>   A   C' ^ USr^~ހwQ gbfeR STUSNeTRS NuNy{|W~ހ0Y!-Nvb~~~ހI{040) 0 DE( 3. r^~ހ] z 1 [IN SOYRyW{Qr^~ހ v^[vQۏLTyd\Ov]zSO| vQvv/fxvzvQuirf[yr'` ^(uNW@xxvzT4N^lu0:G  333J     + F) 2 xvzV 1 Ry0~S0KQ0OX[r^~ހ ^zr^~ހ| 2 xvzr^~ހvuirf[yr'` Yb_`0u0h0RSI{yr'`Nĉ_ 3 PRr^~ހ xvz~ހRSb_b~~SSuirf[ 4 r^~ހNlWV 5 4N^^(u luuu ZnZ 33ff ff f       !          2 ڀ΀r^~ހvyr'` ~ހhQ'`(cell totipotency) ~ހ@bwQ gv1uNy~ހRSb_buir@b gv~~~ހSbukz(WQ vR0 a$ wQ gePXkvR0~ " -"  f-B$ '   R3 )R(u~ހ8hy iڀ΀Ryڀ΀r^~ހ \bSO~~~ހbڀ΀~ހ0ڀ΀r^~ހl\eQS8huSk~ހ-----o;m T---SOYW{Qb_begڀ΀----SOYW{QbVڀ---- mSQ~ހV ----RyQQ~ހV~ހ ----cyNb~~~ހr{QB\W{Q---ڀ΀r^~ހƖ=----cQ*gRSUSKQ~ހV---- yce -----QcyibX0zPP3f    ,                              0 0OQ  `$\ ΀?QvSNYt eY{kY Zk (u75%R|mky (uYyKb/ghVhẽSQ΀?Q nNeAPBS-N Sd4Y0VSQ (ueAPBSmm3^5!k Sd@m Q(u#f:'L                                                 !                            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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz|}~ *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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This information is helping parents choose which embryos they want--and which to reject as unhealthy, or merely undesirable. $! 2  Undesirable Embryos(  iDisease Free Embryos Frozen in storage Donated to infertile couples Donated to stem cell research/usage ,Tji   7Disease Carrying Embryos Donated to research Discarded  87  l,{ NR \ONN` 33  N0(WQ Nd"} [~bRirڀ΀r^~ހxvzgebgvVGr0 N0N\~:NUSMO gDe g'`A~k/f6qۏSv~g KQ/f&T,g1\/fP $FE F ,{VR T 33  10[\uSOYS|b/gxvzۏU\0lSQNyf[ 2008/02 20 N TqQW{QSO~ހT΀[r@nSm^[[rSOYS|Tegڀ΀vq_T0-NVIߘRir 2007/04 30~T[rSOYS|vxvz0SQgyb'Yf[ 2005 40q_TTsNRirKQHesV }xvzۏU\0[_QNyf[ 2007/07 50EmgRirKQb/g0 Nwm\ugr}Q;S 2006/06 60TsNRirKQb/gSvQxvzۏU\0ў_l\ugr}Q;S 2003/10 70r^~ހuirf[yr'`S4N^^(uvxvzۏU\0 Ym_lw;Sf[yf[bf[b 2006/03@       &                $    80__T~SӚW(r^~ހvel0 NwmN'Yf[f[b(;Sf[Hr) 2008/02 90Ӛr^~ހ[TRS:N7h~ހvxvzۏU\0NLuNSNmSBg_ 2008/06 100TsNRir'`+Rc6Rv;Nb/g0INN\ugr 2008/01 110X0Y|P[Ry'`+Rc6Rb/gxvzۏU\0 Nwm\ugr}Q;S 2007/02 120vY[r'`+Rc6Rb/g0sN\ugr}Q;S 2007/05 130Vڀl\lWVES~ހ6R\OL]TSOvxvz0\ugr}Q;Sf[b 2007/02 140q_T~ހ8hy iHesvV }0sN\ugr}Q;S 2008/02 N  l                                 " ,gN@bǑ(uvVGrdRegꁉ[zYec]v[[Y]hl vQ['YRǑƖNQ~ vQ-NRVGr]hlQvQQY ؏ gRVTySV*ghlQY g NS_KNYle (Wdk N6R\O[@b gVGrvHrCgb gh:ya"nZmn " ,gN@bǑ(uvVGrdRegꁉ[zYec]v[[Y]hl vQ[vVGr'YRǑƖNQ~ vQ-NRVGr]hlQvQQY ؏ gRVTySV*ghlQY g NS_KNYle (Wdk N6R\O[@b gVGrvHrCgb gh:ya"qZpq @8 __WPP6pOp r"==>1W)(   &*gbqv[ruSk~ހ(>f_dZPN]\O:yaV"{^opGr 112"2-~ހgڀ΀0NLuOSO~ހKQ Y   [rڀ΀r^~ހ~*F:\[^\2008t^w~|T z3ub\080709\[\uA~kf[ 5gan 9e\,{ASz RirA~kuirf[\ڀ΀r^~ހSvQW{Q.wmv$6/upimg/070628/15_172125.jpgdhttp://www.foodmate.net/upimg/070628/15_172125.jpg(PREV/ 0DTimes New RomanTT_ ܖ 0ܖD[SOes New RomanTT_ ܖ 0ܖ D|^Wes New RomanTT_ ܖ 0ܖ10De[SOs New RomanTT_ ܖ 0ܖ1@DArialNew RomanTT_ ܖ 0ܖ"PDGaramond RomanTT_ ܖ 0ܖ`DWingdingsRomanTT_ ܖ 0ܖpDўSOgdingsRomanTT_ ܖ 0ܖDSymbolgsRomanTT_ ܖ 0ܖDwiSO_GB2312RomanTT_ ܖ 0ܖ1Ff . @n?" dd@  @@``_@,whoosh.wav.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~! 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(trans¶öĶŶƶǶȶɶʶ˶̶Ͷζ϶жѶҶӶԶնֶ׶ضٶڶ۶ܶݶ޶߶fer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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This information is helping parents choose which embryos they want--and which to reject as unhealthy, or merely undesirable. $! 2  Undesirable Embryos(  iDisease Free Embryos Frozen in storage Donated to infertile couples Donated to stem cell research/usage ,Tji   7Disease Carrying Embryos Donated to research Discarded  87  l,{ NR \ONN` 33  N0(WQ Nd"} [~bRirڀ΀r^~ހxvzgebgvVGr0 N0N\~:NUSMO gDe g'`A~k/f6qۏSv~g KQ/f&T,g1\/fP $FE F ,{VR T 33  10[\uSOYS|b/gxvzۏU\0lSQNyf[ 2008/02 20 N TqQW{QSO~ހT΀[r@nSm^[[rSOYS|Tegڀ΀vq_T0-NVIߘRir 2007/04 30~T[rSOYS|vxvz0SQgyb'Yf[ 2005 40q_TTsNRirKQHesV }xvzۏU\0[_QNyf[ 2007/07 50EmgRirKQb/g0 Nwm\ugr}Q;S 2006/06 60TsNRirKQb/gSvQxvzۏU\0ў_l\ugr}Q;S 2003/10 70r^~ހuirf[yr'`S4N^^(uvxvzۏU\0 Ym_lw;Sf[yf[bf[b 2006/03@       &                $    80__T~SӚW(r^~ހvel0 NwmN'Yf[f[b(;Sf[Hr) 2008/02 90Ӛr^~ހ[TRS:N7h~ހvxvzۏU\0NLuNSNmSBg_ 2008/06 100TsNRir'`+Rc6Rv;Nb/g0INN\ugr 2008/01 110X0Y|P[Ry'`+Rc6Rb/gxvzۏU\0 Nwm\ugr}Q;S 2007/02 120vY[r'`+Rc6Rb/g0sN\ugr}Q;S 2007/05 130Vڀl\lWVES~ހ6R\OL]TSOvxvz0\ugr}Q;Sf[b 2007/02 140q_T~ހ8hy iHesvV }0sN\ugr}Q;S 2008/02 N  l                                 " ,gN@bǑ(uvVGrdRegꁉ[zYec]v[[Y]hl vQ[vVGr'YRǑƖNQ~ vQ-NRVGr]hlQvQQY ؏ gRVTySV*ghlQY g NS_KNYle (Wdk N6R\O[@b gVGrvHrCgb gh:ya"qZpq @8 __WPP6pOp ruVCQVCD1W)(   &*gbqv[ruSk~ހ(>f_dZPN]\O:yaV"{^opGr 112"2-~ހgڀ΀0NLuOSO~ހKQ Y   [rڀ΀r^~ހ~*F:\[^\2008t^w~|T z3ub\080709\[\uA~kf[ 5gan 9e\,{ASz RirA~kuirf[\ڀ΀r^~ހSvQW{Q.wmv$6/upimg/070628/15_172125.jpgdhttp://www.foodmate.net/upimg/070628/15_172125.jpg(PREV/ 0DTimes New RomanTT_ ܖ 0ܖD[SOes New RomanTT_ ܖ 0ܖ D|^Wes New RomanTT_ ܖ 0ܖ10De[SOs New RomanTT_ ܖ 0ܖ1@DArialNew RomanTT_ ܖ 0ܖ"PDGaramond RomanTT_ ܖ 0ܖ`DWingdingsRomanTT_ ܖ 0ܖpDўSOgdingsRomanTT_ ܖ 0ܖDSymbolgsRomanTT_ ܖ 0ܖDwiSO_GB2312RomanTT_ ܖ 0ܖ1Ff . @n?" dd@  @@``_@,whoosh.wav.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~! 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N )R(uڀ΀RrRlKQRir(Cloning animal by embryo splitting ) 1(uڀ΀RrRlKQRirW,gi_ 1\/f)R(u:ghRrRvel\N*Nڀ΀RrR:Nr^I{N ORrRvڀ΀Sb*NSOvǏ z0 Ts33&7V '      )  07< 07< `2 Ytڀ΀(deal with Embryo)  \ڀ΀(u ˆ}vvYt oSf&^0 `1fPPJ       p3 RrRڀ΀(splitting Embryo)(u:ghRrRlR \ڀ΀Q~ހVbuSˆtRrR:Nr^R R8fB       4 y i~ހNf&^Q(transfer embryonic cell into pellucid zone)  \Y*NuSˆt~ހbQ~ހV~ހy ieQSڀ΀~ހvf&^-N0 :^ f,%J    -    5 W{Qڀ΀(culture embryos) Ǒ(uSSOW{QlbSOYW{QlW{Qڀ΀0SSOW{Ql1\/f\ڀ΀>e(WRiruS{bP[[҉ )R(uRirSOQagNW{Qڀ΀0rSZf#ffJ     4  v6 y iڀ΀(transfer embryos) \RrRڀ΀y i~~ TgS`YtvSSORirvP[[҉buSQ0B<fJ       7 SSORirY ZTR)ZThe pregnancy of receptor and parturition  y ivKQڀ΀(WSSORirSOQS b_bKQRir02U f-J    )   L 4 1978t^ Moustafa\\ Qh9iڀRrR _ TuSSuTN 5 80t^N Willadsen[~egڀ΀ۏLRrR _VRKNN0kQRKNNRrRڀ΀TN0 6 1986t^ 1uz_Yec[bvbV,{N*Nڀ΀RrR[r(WSQN'Yf[ދuVRڀ 0 7 1988t^ 1u _mYec[bvbV,{N*Nڀ΀RrRNRڀ\ (WSQN'Yf[ދu0vMR] g*s0l0[r00TQ0\ 0NRrRڀTN0 ~Zff)f$f;                  &    <  5vMRX[(WvThe problem of animal cloning by splitting embryos at present 1 Ru͑NOLow of birth weight 2 W ON'`genetic identical 3 @bKQRirpeϑ\a few of clone animal by splitting embryo 4 HQ)Y'`xub_ S No 33@7    =             *      ! &2.~ހ8hy ivaIN: 1 'YE^^cؚoyRirA~kHes RrRbmSegڀ΀ۏLy i KQY*NP[N*NSO 2 :NYnWV[eQ~ހbWVred R agNxvzWVvR0 3 :NۏNekxvz~ހ8h(sQ|`Y[W@x 4 RRiryۏ z MQ6qagN N yRiryhTgTuHesNOvP6R0 f fffffff     *       % "#$%NLuOSO~ހKQ Y    &'( 6(uN~ހ8hy iv^hQ'`~ހ 1 v Nv~ހ(epithelia cell) 2 b~~~ހ(fibroblastic cell) 3 |~ހ(cumulus cell)x]fff                0.3 0.3)O j4 sNz Nv~ހ(mammary epithelia cell) 5 ^y~~ހnerve cell X6ff l          *+ 8.~ހ8hy iKQRirvb/gpThe key methord of animal cloning by cell nuclear transplantation 1 RyTW{QOSO~ހ separating and culturing donor cell 2 SuSk~ހObtain oocyte 3 SOYW{QuSk~ހoocyte culturing in vitro 4 YtOSO~ހ (deal with donor cell)P$D f' f f f  A     #                9.~ހ8hy ielڀ΀~ހ8hy i:NO 1 i_ v^\vQl\eQS8huSk~ހ OvQNS8hgrSO vuSk~ހT SOYSbegڀ΀T yeQSSONuTNvb/g y:Nڀ΀~ހ8hy i0V:N/fe'`A~k SyKQ0RlG$G33PCCJ     M  B. 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